World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Application of a Qpcr Assay in the Investigation of Susceptibility to Malaria Infection of the M and S Molecular Forms of an. Gambiae S.S. in Cameroon, Volume 7

By Snounou, Georges

Click here to view

Book Id: WPLBN0003933963
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Application of a Qpcr Assay in the Investigation of Susceptibility to Malaria Infection of the M and S Molecular Forms of an. Gambiae S.S. in Cameroon, Volume 7  
Author: Snounou, Georges
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Description
Description : Plasmodium falciparum is the causative agent of malaria, a disease that kills almost one million persons each year, mainly in sub-Saharan Africa. P. falciparum is transmitted to the human host by the bite of an Anopheles female mosquito, and Anopheles gambiae sensus stricto is the most tremendous malaria vector in Africa, widespread throughout the afro-tropical belt. An. gambiae s.s. is subdivided into two distinct molecular forms, namely M and S forms. The two molecular forms are morphologically identical but they are distinct genetically, and differ by their distribution and their ecological preferences. The epidemiological importance of the two molecular forms in malaria transmission has been poorly investigated so far and gave distinct results in different areas. We have developed a real-time quantitative PCR (qPCR) assay, and used it to detect P. falciparum at the oocyst stage in wild An. gambiae s.s. mosquitoes experimentally infected with natural isolates of parasites. Mosquitoes were collected at immature stages in sympatric and allopatric breeding sites and further infected at the adult stage. We next measured the infection prevalence and intensity in female mosquitoes using the qPCR assay and correlated the infection success with the mosquito molecular forms. Our results revealed different prevalence of infection between the M and S molecular forms of An. gambiae s.s. in Cameroon, for both sympatric and allopatric populations of mosquitoes. However, no difference in the infection intensity was observed. Thus, the distribution of the molecular forms of An. gambiae s.s. may impact on the malaria epidemiology, and it will be important to monitor the efficiency of malaria control interventions on the two M and S forms.

 

Click To View

Additional Books


  • Plos One : Mir-124 Suppresses Growth of ... (by )
  • Plos One : Twist1 Controls Lung Vascular... (by )
  • Plos One : Hcv Ns5A Protein Containing P... (by )
  • Plos One : Demographic Variation Between... (by )
  • Plos One : Placebo Effects on the Immune... (by )
  • Plos One : Vaccination Rates Among the G... (by )
  • Plos One : Gene Expression Profile of Pe... (by )
  • Plos One : Molecular Chaperone Mediated ... (by )
  • Plos One : More Fair Play in an Ultimatu... (by )
  • Plos One : Meg Network Differences Betwe... (by )
  • Plos One : Activity of Lipase and Chitin... (by )
  • Plos One : Differential Translation of D... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.