World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Genetics : Interallelic and Intergenic Incompatibilities of the Prdm9 Hst1 Gene in Mouse Hybrid Sterility, Volume 8

By Nachman, Michael W.

Click here to view

Book Id: WPLBN0003941874
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Genetics : Interallelic and Intergenic Incompatibilities of the Prdm9 Hst1 Gene in Mouse Hybrid Sterility, Volume 8  
Author: Nachman, Michael W.
Volume: Volume 8
Language: English
Subject: Journals, Science, Genetics
Collections: Periodicals: Journal and Magazine Collection, PLoS Genetics
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Nachman, M. W. (n.d.). Plos Genetics : Interallelic and Intergenic Incompatibilities of the Prdm9 Hst1 Gene in Mouse Hybrid Sterility, Volume 8. Retrieved from http://worldlibrary.org/


Description
Description : The Dobzhansky-Muller model of incompatibilities explains reproductive isolation between species by incorrect epistatic interactions. Although the mechanisms of speciation are of great interest, no incompatibility has been characterized at the gene level in mammals. The Hybrid sterility 1 gene (Hst1) participates in the arrest of meiosis in F1 males of certain strains from two Mus musculus subspecies, e.g., PWD from M. m. musculus and C57BL/6J (henceforth B6) from M. m. domesticus. Hst1 has been identified as a meiotic PR-domain gene (Prdm9) encoding histone 3 methyltransferase in the male offspring of PWD females and B6 males, (PWD6B6)F1. To characterize the incompatibilities underlying hybrid sterility, we phenotyped reproductive and meiotic markers in males with altered copy numbers of Prdm9. A partial rescue of fertility was observed upon removal of the B6 allele of Prdm9 from the azoospermic (PWD6B6)F1 hybrids, whereas removing one of the two Prdm9 copies in PWD or B6 background had no effect on male reproduction. Incompatibility(ies) not involving Prdm9B6 also acts in the (PWD6B6)F1 hybrids, since the correction of hybrid sterility by Prdm9B6 deletion was not complete. Additions and subtractions of Prdm9 copies, as well as allelic replacements, improved meiotic progression and fecundity also in the progeny-producing reciprocal (B66PWD)F1 males. Moreover, an increased dosage of Prdm9 and reciprocal cross enhanced fertility of other sperm-carrying male hybrids, (PWD6B6-C3H.Prdm9)F1, harboring another Prdm9 allele of M. m. domesticus origin. The levels of Prdm9 mRNA isoforms were similar in the prepubertal testes of all types of F1 hybrids of PWD with B6 and B6-C3H.Prdm9 despite their different prospective fertility, but decreased to 53% after removal of Prdm9B6. Therefore, the Prdm9B6 allele probably takes part in posttranscriptional dominant-negative hybrid interaction(s) absent in the parental strains.

 

Click To View

Additional Books


  • Plos Genetics : Progressive Purkinje Cel... (by )
  • Plos Genetics : Mammalian Btbd12 Slx4 Pr... (by )
  • Plos Genetics : Negative Regulation of N... (by )
  • Plos Genetics : Activation of Mutant Enz... (by )
  • Plos Genetics : the Translation Initiati... (by )
  • Plos Genetics : Temporal Regulation of F... (by )
  • Plos Genetics : Wnt16Influences Bone Min... (by )
  • Plos Genetics : Phosphorylation of the D... (by )
  • Plos Genetics : Global Analysis of Extra... (by )
  • Plos Genetics : Four Linked Genes Partic... (by )
  • Plos Genetics : the Paramecium Germline ... (by )
  • Plos Genetics : Dosage-sensitive Functio... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.